Library size against detected genes PDF PNG
Expression distribution PDF PNG
Sample metadata
Sample detail
Quality metrics PDF PNG
Library composition PDF PNG
Donor sex PDF PNG
Sample correlation PDF PNG
Expression, log2 CPM PDF PNG
PC1 against PC2 PDF PNG
Variance explained PDF PNG
Genes driving this component PDF PNG
Downloads
RangedSummarizedExperiment (.rds)
Counts, metadata and gene annotation in one R object.
log2 CPM matrix (.csv.gz)
Genes by samples, the values every view plots.
Sample metadata (.csv)
Every colData column, list columns flattened.

The session as a script or a notebook. All three run on recount3 and ggplot2 alone, with nothing from this app.
Download the session

                
About Recount Explorer

Recount Explorer is a Shiny app for reading the recount3 collection of RNA-seq studies. recount3 holds hundreds of thousands of human and mouse samples, and one pipeline processed all of them in the same way, so counts from different studies are comparable.

Reading one of those studies normally means installing Bioconductor, learning the recount3 API, and writing R. This app removes that step. Pick a study, look at it, and take the data with you.

What you can do

  • Browse all 18,998 studies at once. One search box covers the accession, the title, and the abstract text, so a search for glioblastoma finds studies whose titles never mention it.
  • Overview the samples: library sizes, detected genes, and the full sample metadata.
  • Genes: plot the expression of any gene, split by any metadata column.
  • PCA on the genes with the highest variance, coloured by metadata.
  • Export the data, the figures, and a script that repeats your session.

What a study costs

recount3 stores one file for each study, and there is no way to ask for less of it. Downloads run at about 100 KB for each sample, and memory at about 30 bytes for each gene in each sample. The app shows both before you load anything, and refuses studies over 500 samples so a shared server stays up. RECOUNT_EXPLORER_MAX_SAMPLES moves that limit.

Most studies are small. Of the 18,998 in the catalog, 18,323 are under 10 MB.

How to cite

Cite the recount3 paper for the data:

Wilks C, Zheng SC, Chen FY, et al. recount3: summaries and queries for large-scale RNA-seq expression and splicing. Genome Biology 22, 323 (2021). doi:10.1186/s13059-021-02533-6

The Export view writes a script that carries this reference in its header, along with the accession and the package versions you used.

Author and licence

Built by Samuel Bharti.

The app is MIT licensed. The source is open, and contributions are welcome. The recount3 project sets the terms for the data itself.

How it works

The study catalog is a snapshot that ships with the app, so the list appears at once and needs no network. Loading a study downloads it from the recount3 servers on a background process, which is why the rest of the app stays usable while it runs.

Computation lives in a Shiny-free layer that is tested on its own.

Read the design notes